DNA

Part:BBa_P10101

Designed by: Traci Haddock-Angelli   Group: Plants   (2016-05-25)
Revision as of 22:25, 4 October 2018 by Ryancoates (Talk | contribs)


35s cauliflower mosaic virus

PhytoBricks part from plasmid pUAP41373

The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the RTBV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.



File:Https://static.igem.org/mediawiki/2018/6/6f/T--Cardiff Wales--PromoterResults.png
Figure 1) See only the GUS and mCherry constructs labelled 35S-OTMV-GUS-NosT, or 35S-OTMV-mCherry-NosT




File:Https://static.igem.org/mediawiki/2018/1/19/T--Cardiff Wales--mCherryPromoterAssayQuantified.png
Figure 2) The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 12
    Illegal BglII site found at 399
    Illegal BglII site found at 1145
    Illegal XhoI site found at 1352
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 12
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1355


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