Project

Part:BBa_K2555003:Design

Designed by: Tingyue Li   Group: iGEM18_BFSUICC-China   (2018-09-20)
Revision as of 14:32, 4 October 2018 by Duhongmei (Talk | contribs) (→‎References)

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pBAD-RBS-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067
    Illegal SapI.rc site found at 1355


Design Notes

L3S2P11 and L3S2P21


Source

E.coli

References

(1)Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472.