Project

Part:BBa_K2555003

Designed by: Tingyue Li   Group: iGEM18_BFSUICC-China   (2018-09-20)
Revision as of 14:29, 4 October 2018 by Duhongmei (Talk | contribs)


pBAD-RBS-sfGFP

BBa_K2555002 contains pBAD (L-arabinose inducing promoter) with RBS and CueR ( a transcription factor that can bind on pcopA of BBa_K2555000). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct. CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 and BBa_K2555000 are used for construction of BBa_K2555004. BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP. The construct is used to indirectly reflect the expression of cueR under different concentration of L-arabinose by measuring green fluorescent intensity with plate reader.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067
    Illegal SapI.rc site found at 1355


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Categories
Parameters
None