DNA
Part:BBa_K2889003
Designed by: Rui Zhou Group: iGEM18_Worldshaper-Wuhan (2018-10-04)
pSB1C3-miR-21-sponge-4s
Our previous project constructed a mir-21 sponge containing marker gene of GFP for the detection and inhibition mir-21 in the cell lines, and offers a non-invasive and highly sensitive approach for early diagnosis and treatment of colorectal cancer in the future. miRNA sponge, which contains multiple target sites complementary to a miRNA of interest, is used to be a inhibitor of miRNA. Here, we proposed miRNA sponge could be a monitor to reflect the change of miRNA expression in cells. After transfection of the miR-21 sponge, sponge RNAs can attract miR-21, inhibiting the expression of eGFP reporter. The expression of miR-21 should be monitored by measuring the expression of eGFP reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 66
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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