Composite

Part:BBa_K2598053:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-04)
Revision as of 09:43, 4 October 2018 by Gaoyuanli1998 (Talk | contribs) (→‎References)


GFP+RFP+BFP under regulation of resource allocator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 757
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
    Illegal AgeI site found at 982
    Illegal AgeI site found at 1094
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


Design Notes

RFP and BFP are reversed to avoid leakage. Strong terminators are added to avoid recombination.


Source

The subparts are provided by Christopher A Voigt

References

Fernandezrodriguez J, Moser F, Song M, et al. Engineering RGB color vision into Escherichia coli.[J]. Nature Chemical Biology, 2017, 13(7):706-708.