RNA

Part:BBa_K2889001:Design

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 09:08, 4 October 2018 by Registry (Talk | contribs)

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pSB1C3-IL7-AS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2194
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1529
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2194
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2194
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
    Illegal AgeI site found at 1375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 127
    Illegal BsaI.rc site found at 1183
    Illegal SapI.rc site found at 429


Design Notes

IL7-AS [located anti-sense to interleukin-7 (Il7) gene; Accession number: NM_000880.3] is a newly discovered lncRNA that can be induced across multiple human and mouse cell types and has been reported to regulate the expression of interleukin-6 (IL6). We want to investigate the funtion of IL7-AS in kidney renal clear cell carcinoma. We cloned the full length of IL7-AS (3079bp) and inserted into pSB1C3 for submitting to IGEM 2018. We also inserted the full length of IL7-AS into pCDNA3.1 to study it's function in kidney renal clear cell carcinoma associated cell lines.


Source

We cloned the full length of IL-AS from RNA of human cells.

References