Translational_Unit

Part:BBa_K2688003

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-08-01)
Revision as of 08:34, 4 October 2018 by Briand (Talk | contribs)


cpg2_tu

This composite part is a full translation unti for the Carboxypeptidase G2 (cpg2) without periplasmic localization signal.

This enzyme cleaves folates into pteroates and glutamate(s). It has broad specificity, acting on folic acid, reduced folate but also folate analogs like methotrexate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal NgoMIV site found at 820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 919


[edit]
Categories
//cds
//cds/enzyme
//chassis/prokaryote/ecoli
//function/degradation
Parameters
ec_num3.4.17.11
ligandsfolates, folate analogues, various polyglutamated small molecules, various short peptides
uniprotP06621