Composite

Part:BBa_K2569031

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-10-04)
Revision as of 07:52, 4 October 2018 by Registry (Talk | contribs)

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PdnaA -NicA2

Expressing this BioBrick in E. coli enables the bacterium to degrade nicotine. Nicotine is one of the most harmful ingredients in cigarette and is the biggest resistance to smoking cessation. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in E. coli can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None