Part:BBa_K2740013

CR1 nifH

CR1 nifH encodes nitrogenase reductase NifH, which is an electron donor to the molybdenum-iron (MoFe) protein, contributing to the electron transport in the nitrogen fixation system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 718
  

Parameter of Protein

Number of amino acids: 288

Molecular weight: 31494.95

Theoretical pI: 4.78

Amino acid composition:
Ala (A)  26    9.0%
Arg (R)  13    4.5%
Asn (N)  15   5.2%
Asp (D)  14   4.9%
Cys (C)   6    2.1%
Gln (Q)  13    4.5%
Glu (E)  28    9.7%
Gly (G)  28    9.7%
His (H)   4    1.4%
Ile (I)    22   7.6%
Leu (L)  27    9.4%
Lys (K)  14    4.9%
Met (M)  11   3.8%
Phe (F)   8     2.8%
Pro (P)   8     2.8%
Ser (S)   9     3.1%
Thr (T)  17    5.9%
Trp (W)  0     0.0%
Tyr (Y)   8    2.8%
Val (V)   17   5.9%
Pyl (O)   0     0.0%
Sec (U)   0    0.0%

 (B)   0         0.0%
(Z)   0       0.0%
(X)   0         0.0%

 

Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C          1372
Hydrogen    H         2213
Nitrogen    N            377
Oxygen      O          435
Sulfur      S              17

Formula: C1372H2213N377O435S17
Total number of atoms: 4414

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12295
Abs 0.1% (=1 g/l)   0.390, assuming all pairs of Cys residues form cystines

 

Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.378, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

 

Instability index:

The instability index (II) is computed to be 39.05
This classifies the protein as stable.

 

Aliphatic index: 92.50

Grand average of hydropathicity (GRAVY): -0.161

Design Notes

Nitrogenase is a complex enzyme system consisting of nine protein components. Additionally, to maintain stoichiometry of these protein components is an essential requirement for nitrogenase biosynthesis and activity. However, there is only one copy of each structure gene present in the nif gene cluster. Therefore, cloning each of these nif genes and setting as independent part can facilitate the regulation of balancing expression ratios from the transcription and/or translation level(s) when they are heterogeneously expressed in non-diazotrophic hosts.