Part:BBa_K2570011
dsrA+lacI+PlacI+hns+ProU+GFP
In our project, we combined temperature promoter dsrA and salt-concentration promoter ProU as a logical gate controlled by salt-concentration and temperature to realize the aim of producing antibacterial, PLA.
dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher. [1]The salt-concentration promoter ProU provides sensitivity to the salt concentration to human skin. ProU is a promoter existed in E.coli, induced by low salt concentration. And the HNS is a global-regulated protein which could bind to genome and specifically inhibit ProU.[2]
In our design, our engineering bacteria usually put in a spray container and store at room temperature. When temperature stays below 37 degree, the temperature promoter will not work.
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The function of PlacI has became a constitutive promoter. The global regulate protein HNS will be express to inhibit proU. Then the logical gate will be “off” state.
On the other hand, when we use the spray on the skin, temperature reached 37 degree, the repressor protein lacI will be expressed to inhibit PlacI, the HNS won’t be revealed. Then, once people sweat, the Na+ and Cl- will induce proU, the logical gate became “on” state.
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1587
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3324
Illegal SapI.rc site found at 154
Illegal SapI.rc site found at 1973
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