Part:BBa_K2865000
BNP promoter, heart failure inducible
This part encodes for the brain natriuretic peptide (BNP) promoter. The use of this part is to express wanted genes specifically in failing hearts, so as to enhance the selectivity of gene therapy of heart failure and minimize its side effects.
Usage and Biology
Brain natriuretic peptide (BNP), also known as B-type natriuretic peptide, is a hormone secreted by cardiomyocytes in the heart ventricles. It has been regarded as an important biomarker in the diagnosis of heart failure (HF), for its level is highly related to the severity of HF. The activity of BNP promoter remains low in normal hearts and elevates dramatically in the cardiomyocytes of patients with HF. Based on the qualities mentioned above, it is natural to think that we can utilize BNP promoter to control gene expression in HF gene therapy. According to previous studies, BNP promoter can be activated by a number of factors associated with HF, including endothelin (ET), angiotensin II (Ang II), mechanical strain, and hypoxia, among others. These regulating activities could be attributed to the combination of a large variety of transcriptional factors and cis-acting elements at the upstream of the promoter. However, little is known about the actual transcriptional mechanisms responsible for their regulation and induction in the heart.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 242
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