Composite

Part:BBa_K2812005:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls   Group: iGEM18_TU-Eindhoven   (2018-10-03)
Revision as of 15:27, 3 October 2018 by Registry (Talk | contribs)

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Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick contains the coding domain for truncated lysostaphin. Lysostaphin targets the cell wall peptidoglycan found in certain Staphylococci by cleaving its cross-linking pentaglycine bridges. Among others, it is effective for degrading S. aureus biofilm. The HlyA tag is a secretion signal peptide which is used for secretion via the Type 1 secretion pathway of gram-negative bacteria. In order to facilitate protein purification, a N-terminal HIS-tag is present. . In this biobrick, lysostaphin production is regulated by the T7 promoter. Expression can be induced by the presence of Isopropyl β-D-1-thiogalactopyranoside (IPTG).


Source

Uniprot

References