Part:BBa_K2812005
Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter
This biobrick contains the coding domain for truncated lysostaphin. Lysostaphin targets the cell wall peptidoglycan found in certain Staphylococci by cleaving its cross-linking pentaglycine bridges. Among others, it is effective for degrading S. aureus biofilm. The HlyA tag is a secretion signal peptide (BBa_K554002) which is used for secretion via the Type 1 secretion pathway of gram-negative bacteria. In order to facilitate protein purification, a N-terminal HIS-tag is present. . In this biobrick, lysostaphin production is regulated by the T7 promoter. Expression can be induced by the presence of Isopropyl β-D-1-thiogalactopyranoside (IPTG).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1389
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 164
- 1000COMPATIBLE WITH RFC[1000]
//cds
//cds/enzyme
//cds/enzyme/lysis
//cds/membrane
//cds/membrane/lysis
//chassis/prokaryote/ecoli
//collections/biofilm
//plasmid/expression
//plasmid/expression/t7
//plasmidbackbone/expression/inducible
//plasmidbackbone/proteinfusion
//plasmidbackbone/synthesis
//proteindomain
//proteindomain/cleavage
//proteindomain/degradation
//proteindomain/linker
//proteindomain/localization
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