Composite
Part:BBa_K2812003
Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls Group: iGEM18_TU-Eindhoven (2018-10-03)
Coding sequence for trunctated Lysostaphin regulated by T7-promoter
This biobrick contains the coding domain for truncated lysostaphin. Lysostaphin targets the cell wall peptidoglycan found in certain Staphylococci by cleaving its cross-linking pentaglycine bridges. Among others, it is effective for degrading S. aureus biofilm. In this biobrick, lysostaphin production is regulated by the T7 promoter. Expression can be induced by the presence of isopropyl β-D-1-thiogalactopyranoside (IPTG).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 164
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
//cds/enzyme
//cds/enzyme/lysis
//cds/membrane
//cds/membrane/lysis
//chassis/prokaryote/ecoli
//collections/biofilm
//plasmid/expression
//plasmid/expression/t7
//plasmidbackbone/expression/inducible
//plasmidbackbone/synthesis
//proteindomain
//proteindomain/cleavage
//proteindomain/degradation
//cds/enzyme
//cds/enzyme/lysis
//cds/membrane
//cds/membrane/lysis
//chassis/prokaryote/ecoli
//collections/biofilm
//plasmid/expression
//plasmid/expression/t7
//plasmidbackbone/expression/inducible
//plasmidbackbone/synthesis
//proteindomain
//proteindomain/cleavage
//proteindomain/degradation
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