Coding
CfaN

Part:BBa_K2549009

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-03)
Revision as of 10:26, 3 October 2018 by JennyDu (Talk | contribs)


split intein Cfa N

This part is the N-terminal fragment of Cfa. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. Besides, when the Cfa’N is fused to other proteins, it exhibits higher expression levels than other N-terminal fusions. Cfa’N is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
None