Reporter

Part:BBa_K2708004:Design

Designed by: LAN FEI   Group: iGEM18_BIT   (2018-10-02)
Revision as of 08:07, 3 October 2018 by Farfrom (Talk | contribs) (→‎Source)

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Promoter withS-adenosyl methionine ribose switch, LacI protein expression system.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The combination of SAM is negatively correlated with the expression of the circuit.


Source

Synthetic promoter plus riboswitch +4-1N(B0034)+2-2N(C0012)+3-3F(B0015) Riboswitch is a kind of RNA sequence element with special spatial structure. It is mainly found in the non-coding region of bacterial mRNA. SAM riboswitch is widely distributed in various bacterial lineages.

References