Composite
Part:BBa_K2669003
Designed by: Matilda Brink Group: iGEM18_Uppsala (2018-10-02)
Revision as of 15:31, 2 October 2018 by MatildaBrink (Talk | contribs)
Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator
Composite containing optimized AmilCP BBa_K2669002, RBS BBa_J34801, constitutive promotor BBa_J23119 and a double terminator BBa_B0014. The part contains a blue chromoprotein which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture.
Part can be used as indicator or reporter and is expressed constitutively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/chromoprotein
abs | 588 |
chassis | E.coli DH5-alpha |
color | Blue |
control | Constitutive |