Composite

Part:BBa_K2671000

Designed by: Oliver Hild Walett   Group: iGEM18_Linkoping_Sweden   (2018-10-02)
Revision as of 13:58, 2 October 2018 by Registry (Talk | contribs)

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AraC-pBAD-mNG

Liu iGEM2017s plasmid with a improvement. A stop codon was mutated behind mNG (mNeonGreen). The original part was mNG fused to amyloid-β, where the fusion protein had its stop codon after amyloid-β. With this improvement mNG can now be used as a reporter in a fully functional operone. Note that the sequence for amyloid-β is still present, it will be transcribed but not translated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1267
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
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