Composite

Part:BBa_K2669003

Designed by: Matilda Brink   Group: iGEM18_Uppsala   (2018-10-02)
Revision as of 09:25, 2 October 2018 by Registry (Talk | contribs)

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Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator

Composite part in pbs1C3 backbone containing optimized AmilCP (BBa_K2669002), RBS (BBa_J34801), constitutive promotor (BBa_J23119) and a double terminator (BBa_B0014). The part contains a blue chromoprotein which naturally exhibits strong color when expressed that can be observed with naked eye in both LB and agar culture.

Part can be used as indicator or reporter and is expressed constitutively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/chromoprotein
Parameters
abs588
chassisE.coli DH5-alpha
colorBlue
controlConstitutive