Coding
Part:BBa_K2748001
Designed by: Xiaowen Mao Group: iGEM18_SYSU-CHINA (2018-10-02)
rtTA-advanced (reverse tet-controlled transactivator)
synthetic transcription transactivator with rtetR (mutant of tetR) from Escherichia coli and VP16 transcription activation domain from herpes simplex virus, has been widely used for transcriptional activation by tetracyclines in mammalian cells (Gossen, M., et al. (1995). "Transcriptional activation by tetracyclines in mammalian cells." Science 268(5218): 1766-1769.)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |