Regulatory

Part:BBa_K2558001

Designed by: Tianze Huang   Group: iGEM18_Tsinghua   (2018-10-02)
Revision as of 07:29, 2 October 2018 by Huangtz (Talk | contribs)


lux pR-HS

This part is derived from lux pR https://parts.igem.org/Part:BBa_R0062. They are similar except for one point mutations in the luxR binding site (G19T) and another three in the -10 region of the promotor (TAG44CTT). The functions remains the same, the mutant luxpR promotor is inducible by LuxR-AHL complex. However they do differ in gene expression strength, leakage and the expression perimeters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controlluxR, HSL
directionForward
negative_regulators
o_h
o_l
positive_regulators1