Coding

Part:BBa_K2609002

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-23)
Revision as of 17:14, 1 October 2018 by BhaskarK (Talk | contribs)


T4 bacteriophage long tail fiber protein gp37 (Modification I)

Coding sequence of the long tail fiber protein from T4 bacteriophage. The sequence has been modified in-silico for better binding to phosphoethanolamine near the receptor binding end of the protein. This is the first such modification in our series of four modifications.


Usage and Biology

The T4 bacteriophage uses its long tail fiber to recognize and bind to its receptor OmpC on the surface of E. coli cells, its cognate host. The binding is mediated by non-covalent interactions that leads to the docking of the tip of the tail fiber (protein gp37).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None