Composite
Kai 1 3

Part:BBa_K2623013

Designed by: Yi Lin   Group: iGEM18_XMU-China   (2018-09-28)
Revision as of 14:48, 1 October 2018 by Bb1984 (Talk | contribs) (→‎SUMMARY)


Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC

SUMMARY

To facilitate the construction of plasmids, we constructed two circuit，both Part:BBa_K2623010 and Part:BBa_K262312 into the same pSB1C3 plasmid.

Fig.1
Fig.2


























































Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 627
    Illegal AgeI site found at 1442
    Illegal AgeI site found at 2153
    Illegal AgeI site found at 2162
    Illegal AgeI site found at 2168
    Illegal AgeI site found at 2492
    Illegal AgeI site found at 2519
  • 1000
    COMPATIBLE WITH RFC[1000]


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