Coding
LaG16

Part:BBa_K2549003

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 12:11, 1 October 2018 by Registry (Talk | contribs)

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anti-GFP (LaG16)

Anti-GFP(LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. It can be used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. When fused with LaG2 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/receptor/antibody
Parameters
None