Composite

Part:BBa_K2587027

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-10-01)
Revision as of 11:33, 1 October 2018 by Yllon (Talk | contribs)


luxI_luxR_Plux_gfp

This construct comprises parts from the Quorum sensing system from Vibrio fischeri. It contains the LuxI, the acyl homoserine lactone synthase, the LuxR, the regulator, which will bind to the promoter pLux, that will induce synthesis of GFP.

Usage and Biology


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 829
    Illegal NheI site found at 852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 892
    Illegal BsaI site found at 1681
    Illegal BsaI.rc site found at 1523
    Illegal BsaI.rc site found at 1790
    Illegal BsaI.rc site found at 2468


[edit]
Categories
Parameters
None