Composite

Part:BBa_K2587027:Design

Designed by: Ylenia Longo   Group: iGEM18_Duesseldorf   (2018-10-01)
Revision as of 09:53, 1 October 2018 by Registry (Talk | contribs)

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luxI_luxR_Plux_gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 829
    Illegal NheI site found at 852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 892
    Illegal BsaI site found at 1681
    Illegal BsaI.rc site found at 1523
    Illegal BsaI.rc site found at 1790
    Illegal BsaI.rc site found at 2468


Design Notes

This part contains Type II S restriction sites and can be used for modular assembly with other parts.


Source

The sequences for LuxI, LuxR and pLux are from GenBank and codon optimised for E.coli. The remaining sequences are from the CIDAR MoClo toolbox.

References