Coding
surCD19

Part:BBa_K2549001

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 05:44, 1 October 2018 by Registry (Talk | contribs)

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suface-expressed CD19

Surface-expressed CD19(surCD19) is built by fusing CD8alpha signal peptide and CD19 extracellular domain which has a HA tag on its N terminal and a Myc tag on its C terminal to the transmembrane region of PDGFRbeta from N terminal to C terminal. CD8alpha peptide guides surCD19 to be located to the membrane. Transmembrane region of PDGFRbeta embeds it on the plasma membrane. The HA tag and the Myc tag facilitate researchers to detect the expression of the surCD19.It was used as one of our antigens to test the activation of SynNotch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757
    Illegal SapI site found at 273
    Illegal SapI.rc site found at 91


[edit]
Categories
//collections/immune_regulation/receptors
Parameters
protein