Coding

Part:BBa_K2599006

Designed by: YEN-LING CHEN   Group: iGEM18_NCTU_Formosa   (2018-09-16)
Revision as of 00:40, 1 October 2018 by Yen-ling (Talk | contribs)


Antimicrobial peptide - Leucocyclicin Q

This biobrick is the basic part of the Leucocyclicin Q, more information please look for the composite part (BBa_K2599014).



Figure 1. Basic part of Leucocyclicin Q


Introduction

Leucocyclicin Q, produced by Leuconostoc mesenteroides TK41401, possesses antimicrobial activity against broad range of bacteria srains, and particularly shows strong activity against Bacillus coagulans. Leucocyclicin Q was proved to be a cyclic bacteriocin in which N and C termini are bound to each other, and its structure is very similar to Lactocyclicin Q. Furthermore, it shows high stability against different pH value or heat stress.

Mechanism of Leucocyclicin Q

The bacteriocins inhibit their target organisms through pore formation. Though the mechanism of each inhibition is vary from species to species, the general process is conserved. To see more details, please search for our project page.


Features of Leucocyclicin Q

1. Species Specific

Bacteriocins are antimicrobial peptides that will kill or inhibit bcterial strains closely related or non-related to produced bacteria, but will not harm the bacteria themselves by specific immunity proteins. The organisims that Leucocyclicin Q targets including Enterococcus faecalis, Bacillus subtilis, Bacillus coagulans, etc. More target organisms can be found on [http://bactibase.hammamilab.org/BAC209 bactibase].

2. Eco-friendly

Since Leucocyclicin Q is a polypeptide naturally produced by bacteria itself and can inhibit other bacteria without much environment impact. It don't pose threat to other organisms like farm animals or humans. Therefore, this toxin will not cause safety problem.

3. Biodegradable

Leucocyclicin Q is a short peptide that will degrade in a short time. After degradation, this antibacterial peptide is harmless to our environment.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Leucocyclicin Q sequence length is around 186 b.p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

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Categories
Parameters
None