Part:BBa_K2587024

luxI_luxR_P_lux_E

This part is an assembly of different constructs, which then yield a final plasmid, able to self lyse Escherichia coli cells. It is a construct based on the Quorum sensing system, which induces cell lysis upon synthesis of the quorum sensing molecule acyl homoserine lactone. Upon synthesis of this by the LuxI gene, it will bind to the regulator LuxR and then to the promoter pLux, which will activate synthesis of the lysis protein E from the bacteriophage phiX174E.

Usage and Biology This construct can be used to regulate cell density. Compared to the wildtype cells, cells harbouring this plasmid grow slower. Observation of their growth shows a remissive exponential phase, reaching the stationary phase with a lower cell density compared to wild type celly.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 829
  Illegal NheI site found at 852
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1082
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 892
  Illegal BsaI site found at 1681
  Illegal BsaI site found at 1832
  Illegal BsaI.rc site found at 1523
  Illegal BsaI.rc site found at 1790