Coding
Part:BBa_K2560260
Designed by: Daniel Marchal Group: iGEM18_Marburg (2018-09-30)
mcr gene for Malonyl-CoA Reductase from Chloroflexus aurantiacus
mcr encoded the enzyme Malony-CoA Reductase from C. aurantiacus. This enzyme is involved in the 3-hydroxypropionate cycle for CO2 fixation and converts malonyl-CoA into 3-hydroxypropionic acid (Strauss & Fuchs, 1993). In a two-step reaction malonyl-CoA is first reduced to 3-oxopropanoic acid and then further reduced to 3-hydroxypropionic acid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1932
Illegal NheI site found at 3334 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 885
Illegal BamHI site found at 213 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1188
Illegal NgoMIV site found at 2005
Illegal NgoMIV site found at 3619
Illegal AgeI site found at 1270
Illegal AgeI site found at 3168 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |