Composite
Part:BBa_K2669000:Experience
Designed by: Joseph Caponi Group: iGEM18_Uppsala (2018-09-25)
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===Applications of BBa_K2669000===
After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found here Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.
Figure 2: Bilirubin test before/after affinity chromatography. Going from right to left the samples are:
- Lysed sample of the “bad” part before AC
- Lysed sample of the “good” part before AC
- "Bad" part after AC
- "Good" part after AC