Part:BBa_K2669000:Experience

__NOTOC__ This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out. ===Applications of BBa_K2669000=== After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found here Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.

Figure 2: Bilirubin test before/after affinity chromatography. Going from right to left the samples are:

• Lysed sample of the “bad” part before AC
• Lysed sample of the “good” part before AC
• "Bad" part after AC
• "Good" part after AC

===User Reviews=== BBa_K2669000 StartReviews BBa_K2669000 EndReviews