Composite

Part:BBa_K2669000:Experience

Designed by: Joseph Caponi   Group: iGEM18_Uppsala   (2018-09-25)
Revision as of 11:39, 30 September 2018 by Jjc0000 (Talk | contribs)

__NOTOC__ This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out. ===Applications of BBa_K2669000=== After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found here Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.

"https://static.igem.org/mediawiki/2018/2/20/T--Uppsala--UnaG_Comparison.png"

Figure 2: Bilirubin test before/after affinity chromatography. Going from right to left the samples are:

  • Lysed sample of the “bad” part before AC
  • Lysed sample of the “good” part before AC
  • "Bad" part after AC
  • "Good" part after AC
===User Reviews=== BBa_K2669000 StartReviews BBa_K2669000 EndReviews