Part:BBa_K2570011
dsrA+lacI+PlacI+hns+ProU+GFP
In our project, we combined temperature promoter dsrA and salt-concentration promoter ProU as a salt-concentration and temperature control promoter o the aim of producing antibacterial, PLA. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher. [1]The salt-concentration promoter ProU provides sensitivity to the salt concentration to human skin. ProU is a promoter existed in E.coli, induced by low salt concentration. And the HNS is a global-regulated protein which could bind to genome and specifically inhibit ProU.[2] When temperature reached 37 degree, suitable for human body, temperature promoter dsrA will express protein lacI. The promoter induced by lacI(PlacI) will be inhibited, the global regulate protein HNS will not be express. Then, people sweat, the salt in the sweat will decide the production of PLA . On the other hand, when our circuits in the container, which does not start to work. Not enough room temperature to induce dsrA, the temperature promoter. The promoter induced by LacI will express global regulate protein HNS, which could repress ProU. To solve promoter leak problem, our membrane provided a salt-free environment to prevent the production of PLA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1587
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3324
Illegal SapI.rc site found at 154
Illegal SapI.rc site found at 1973
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