Coding

Part:BBa_K2729009

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)
Revision as of 05:58, 30 September 2018 by Registry (Talk | contribs)

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AmCyan_FMDV2a

AmCyan is one of the existing violet fluorochromes for use in flow cytometers equipped with a violet (405 nm) laser. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 820
    Illegal AgeI site found at 669
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None