Coding

Part:BBa_K2729008

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)
Revision as of 05:57, 30 September 2018 by Registry (Talk | contribs)

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ZsYellow-FMDV 2A

YFP is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 820
    Illegal AgeI site found at 632
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None