Composite

Part:BBa_K2878006

Designed by: Yilin Mo   Group: iGEM18_SSHS-Shenzhen   (2018-09-30)
Revision as of 05:56, 30 September 2018 by Registry (Talk | contribs)

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ARK-shRNA-1 template with T7 promoter

This DNA oligo consists of T7 promoter (TAATACGACTCACTATA) and ARK-shRNA-1 transcription template (GGAGTTCGATGCCGTTAAGGAGTTCGTTGTCCTTAACGGCATCGAACTCCTT), We use this DNA oligo for ARK-shRNA-1 generation through in vitro transcription system. With the DNA oligo of ARK-shRNA-1 template with T7 promoter, we add T7 RNA polymerase, NTP and in vitro transcription buffer, ARK-shRNA-1 can be generated within 30 minutes. ARK-shRNA-1 is used to silence Phyllotreta striolata Arginine Kinase gene, the target site on the Arginine Kinase mRNA is 997-1018 after AUG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None