Coding

Part:BBa_K2729007

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)
Revision as of 05:54, 30 September 2018 by Registry (Talk | contribs)

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DsRed-Express-FMDV 2A

DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green fluorescent protein (GFP) from the jellyfish Aequorea. The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 808
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None