Tag

Part:BBa_K2560258:Design

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-29)
Revision as of 21:58, 29 September 2018 by Marchal (Talk | contribs)


Streptag for C-terminal protein tagging


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).

If the Streptag shall be fused to the 3' end (C-terminus of the protein), use this part, for 5' fusion (N-terminus of the protein) use BBa_K2560257.

GGTCTCGGCTTTA-coding_region-GGGTATGAGACC

The sequence was codonoptimized for V. natriegens ATCC 14048.

Source

Source of the part:

The sequence of this part was used from Addgene (Plasmid #55181). To stay in frame, a TA was added before the coding region and a G behind it. (LWSHPQFEKG)

The part was codonoptimized for V. natriegens and then synthetisized by IDT and integrated into the vector BBa_K2560002via BsmBI

References

Weber E., Engler C., Gruetzner R., Werner S., Marillonnet S. (2011). A modular cloning system for standardized assembly of multigene constructs. PLoS One 6:e16765. 10.1371/journal.pone.0016765