Regulatory

Part:BBa_K2560007

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-08-20)
Revision as of 14:55, 29 September 2018 by Henselt (Talk | contribs)


Phytobrick version of BBa_J23100

More information coming soon!

Information category Part (marburg toolbox) in the right corner overview about the usage (marburg toolbox) characterisation parts (experiments etc.)


<img src="T--Marburg--Promoterstrength.jpeg" width="400px"/>

Figure 1. Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression. Induced at time=0h.

Usage and Biology

This is the Phytobrick version of the promoter BBa_J23100. Marburg 2018 characterized this part in Vibrio natriegens using the lux operon of Photorhabdus luminescens (BBa_K2560051). The messured relative promotor strenghts in Vibrio natriegens were correlated to the relative promotor strenghts in E. coli (iGEM Berkeley 2006). The parts sequence was verified by Sanger sequencing.

Plasmid map of the test construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None