![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2865001:Design
Designed by: Chuqi Wang Group: iGEM18_SMMU-China (2018-09-29)
AR185-T2A-EGFP, nanobody inhibiting RyR2 phosphorylation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 155
Illegal NgoMIV site found at 302 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
RyR2-specific nanobodies were isolated from a phage display library of variable domains of camellidae heavy chain-only antibodies (VHH). AR185, one of the isolated nanobodies, inhibiting RyR2 phosphorylation in an in vitro assay was then chosen.
Source
This nanobody was acquired through phage display and biopanning.