Coding

Part:BBa_K2865001

Designed by: Chuqi Wang   Group: iGEM18_SMMU-China   (2018-09-29)
Revision as of 13:31, 29 September 2018 by Registry (Talk | contribs)

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AR185-T2A-EGFP, nanobody inhibiting RyR2 phosphorylation

AR185 is a camel single-domain antibody to cardiac ryanodine receptor2 (RyR2) that have the ability to inhibit PKA dependent Ryr2 phosphorylation. This part BBa_K2865001 is combined by nanobody AR185 and reporter eGFP by self-cleaving peptide T2A.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 155
    Illegal NgoMIV site found at 302
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None