Composite
SAHS 1

Part:BBa_K2623015

Designed by: Jun Dai   Group: iGEM18_XMU-China   (2018-09-28)
Revision as of 13:19, 29 September 2018 by Bb1984 (Talk | contribs)


Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator)

TDP circuit is used to express exocrine protein SAHS. In order to facilitate the characterization and purification of SAHS protein, we added the promoter J23100+RBS+SAHS protein+RBS+E1010+B0015 to pSB1C3. TDP circuit is used to express exocrine protein SAHS BBa_K2623007. We added RFP BBa_E1010 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100 

and an RBS BBa_B0034 , and followed by a double terminator BBa_B0015.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 279
    Illegal XhoI site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1152
    Illegal AgeI site found at 1264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 558
    Illegal SapI.rc site found at 553


[edit]
Categories
Parameters
None