Composite

Part:BBa_K2666008

Designed by: Léa Meneu   Group: iGEM18_Montpellier   (2018-09-29)
Revision as of 07:47, 29 September 2018 by Registry (Talk | contribs)

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RFP with B.subtilis specific strong promoter R0

The Montpellier iGEM team 2018 designed a construction that produce RFP in Bacillus subtilis (more information). For that, we used the promoter R0, a B.subtilis specific strong promoter [1]. Figure 1 illustrates the detailed design.

Figure 1 : Construct design. The sequence that encode RFP fusionned with a strong promoter R0.

The objective is to check the strength of several construction in E.coli, L.jensenii & B.subtilis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 62
    Illegal BamHI site found at 194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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