Coding
Part:BBa_K2560253:Design
Designed by: Daniel Marchal Group: iGEM18_Marburg (2018-09-28)
birA from C. glutamicum, codonoptimized for V. natriegens
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 132
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
Source of the part:
Genome: Corynebacterium glutamicum ATCC 13032
Accession number of gene: NCgl0679
Accession number of encoded protein: NP_599941.1
birA was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI