Coding

Part:BBa_K2560253

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-28)
Revision as of 18:49, 28 September 2018 by Registry (Talk | contribs)

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birA from C. glutamicum, codonoptimized for V. natriegens

birA encodes an biotin-ligase in C. glutamicum. It transfers a biotin to the biotin-carboxylase domain (also known as biotin-carboxyl-carrier protein BCCP) of several biotin-dependent enzymes like Acetyl-CoA Carboxylase and Propionyl-CoA Carboxylase (Samols et al., 1988). Hence this enzyme is involved in fatty acid biosynthesis. For this part, accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI. If birA shall be used with an 5' tag use Bba_K2560256, if there is no need for tagging, use this part.


Samols. D.. Thornton, C.H., Murtif. V.L.. Kumar, G.K.. Haase. F,C. and Wood. H.G. (1988) J. Biol. Chem. 263. 6461-6464.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None