Composite
Kai 2

Part:BBa_K2623011

Designed by: Gezhi Xiao   Group: iGEM18_XMU-China   (2018-09-28)
Revision as of 18:31, 28 September 2018 by Registry (Talk | contribs)

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Phosphorylation pathsway builder of Kai

We added the promoter J23100+RBS+CikA+Rpa+SasA+B0015 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 188
    Illegal BglII site found at 3666
    Illegal BglII site found at 3735
    Illegal BamHI site found at 2248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None