Composite
Kai 2
Part:BBa_K2623011
Designed by: Gezhi Xiao Group: iGEM18_XMU-China (2018-09-28)
Phosphorylation pathsway builder of Kai
We added the promoter J23100+RBS+CikA+Rpa+SasA+B0015 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 188
Illegal BglII site found at 3666
Illegal BglII site found at 3735
Illegal BamHI site found at 2248 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |