Coding

Part:BBa_K2560255:Design

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-28)
Revision as of 18:09, 28 September 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


accD subunit from C. glutamicum, codonoptimized for V. natriegens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the accBC gene of C. glutamicum (NCgl0678) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsmBI recognition sites (Weber et al., 2011). If accBC shall be used with an 5' tag use BBa_K2560254, if there is no need for tagging, use this part.AACGTCTCGCTCGAATG-coding_region-GCTTTGAGGGAGACGAAThe sequence was codonoptimized for V. natriegens ATCC 14048.



Source

Source of the part: Genome: Corynebacterium glutamicum ATCC 13032Accession number of gene: NCgl0678Accession number of encoded protein: NP_599940.1accD was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI


References