Part:BBa_K2688027

LEE5_GFP_native_ΔTir

LEE (locus of enterocyte effacement) is a genomic island that encodes a secretion system in enteropathogenic E. coli. This BioBrick is a small part of the island containing the promoter region of the 5th operon, and biobricks to express a generic GFP.

Compared to LEE5_native (BBa_K2688010), this variant has a deletion that removes the tir fragment. (The beginning of an ORF is present in the native sequence : it is removed for improved expression and to avoid clash of reading frame)

This region has been shown to be recognized by two nucleoid-associated proteins, H-NS and Ler (‘LEE encoded regulator’). The regulation by these proteins can generate some heterogeneity of transgene expression within E. coli populations as recently shown by Leh and collaborators (Leh et al, mBIO, 2017).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 50
  Illegal BsaI.rc site found at 1003