Coding

Part:BBa_K2560251

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-26)
Revision as of 15:35, 28 September 2018 by Marchal (Talk | contribs)


accBC subunit from C. glutamicum, codonoptimized for V. natriegens

Acetyl-CoA Carboxylase subunits

accBC encodes the biotin carboxylase domain of the acetyl-CoA-carboxylase (Acc) in C. glutamicum. The enzyme is involved in fatty acid biosynthesis by converting acetyl-CoA into malonyl-CoA. In C. glutamicum the enzyme consists of two subunits, a biotin-carboxylase and a carboxytransferase. To get activ the enzyme has to be biotinylated what is made by an biotin-ligase (BirA).

For this part, accBC was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.

If accBC shall be used with an 5' tag use BBa_K2560254, if there is no need for tagging, use this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 423
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None